Apoptosis Biochemical Screening
Apoptosis Biochemical Screening | Creative Biogene
Apoptosis can be triggered by two known pathways: the intrinsic pathway (also called the mitochondrial pathway) and the extrinsic pathway. The intrinsic pathway, as its name implies, is activated by intracellular stimuli such as irreparable genetic damage, hypoxia, extremely high concentrations cytosolic Ca2+ and severe oxidative stress. This pathway is closely regulated by a group of proteins belonging to the Bcl-2 family and is the result of increased mitochondrial permeability and the release of pro-apoptotic molecules such as cytochrome c into the cytoplasm. The other pathway, extrinsic pathway is initiated when extracellular ligands binding to cell-surface death receptors. The best known death receptors are the type 1 TNF receptor (TNFR1) and a related protein called Fas (CD95) and their ligands are called TNF and Fas ligand (FasL) respectively.