Recombineering based Microbe Genome Editing
Recombineering based Microbe Genome Editing | Creative Biogene
Creative Biogene offers microorganism genome mutation, insertion, gene knock out and metabolic pathways engineering service for a broad range of microbes by our mature recombineering platform. Our microbe targets are including but not limited to Escherichia coli, Streptomyces, Mycobacterium, and Candida albicans, and so on.
Recombinase-mediated homologous recombination, also known as recombineering, has revolutionized bacterial genetics, synthetic biology, and metabolic engineering. Relying on either RecET from the Rac prophage or the bacteriophage lambda Red proteins, Exo, Beta, and Gam, recombineering allows simple and efficient construction of gene knockout mutants via homologous recombination of a double stranded DNA (dsDNA) polymerase chain reaction (PCR) product with bacterial chromosomes.